siRNA做基因敲低时，为什么都是强调mRNA水平的检测，蛋白水平不能反映siRNA的效果吗？
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登录后方可回帖
因为siRNA的作用机制是通过RNA干扰（RNAi）来降解目标基因的mRNA分子，所以mRNA水平是最直接的指标。mRNA翻译为蛋白质的过程中会经历各种复杂的修饰和调控，进而出现mRNA和蛋白水平变化不一致的现象。另一方面，蛋白本身的稳定性（如半衰期）也是影响蛋白水平变化的重要因素。还有可能涉及细胞的补偿机制等等复杂的生物学效应。因此蛋白水平不能直接反映siRNA的干扰效果。